PGC-1α over-expressing animals have higher mitochondrial content and are protected from denervation-induced muscle atrophy. (A) TA muscle mass corrected for body weight. (B) PGC-1α blot confirming overexpression in TA muscle. (C) Representative blot and quantification of COXIV as a mitochondrial content marker. (D) Representative images of SDH staining of TA muscle from Con and Den WT and Tg animals. *P < 0.05, significant effect of denervation. †P < 0.05, significant effect of genotype (n = 3 to 5 for all groups). Con, control; Den, denervated; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PGC-1α, peroxisome proliferator co-activator 1 alpha; TA, tibialis anterior; Tg, transgenic; WT, wild type.