Figure 6
From: Oxidative stress, mitochondrial damage, and cores in muscle from calsequestrin-1 knockout mice
Increased mitochondrial volume and mitochondrial damage in EDL muscles from 4- to 6-month-old CASQ1-null mice. (A, B) Representative EM images showing increased mitochondrial number (empty arrows) in fibers from 4- to 6-month-old CASQ1-null mice (B) compared to WT mice (A) (see also ref. [25]). (C) Frequency distribution of the relative percentage of fiber volume occupied by mitochondria in WT and CASQ1-null fibers. Average (±SEM) percent mitochondrial volume is shown above each distribution (P < 0.01). (D-G) Representative mitochondria presenting structural abnormalities (D-F), which are more frequent in fibers from CASQ1-null mice compared to that of WT mice (G). Number of WT and CASQ1-null animals tested: n = 3. Number of fibers analyzed: n = 5. Data given as percentage ± SEM (**P < 0.01). Scale bars, (A, B): 0.2 μm; (D, E) and (G): 0.1 μm.EDL, extensor digitorum brevis; EM, electron microscopy CASQ1, skeletal isoform of calsequestrin; WT, wild type.