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Figure 7 | Skeletal Muscle

Figure 7

From: Oxidative stress, mitochondrial damage, and cores in muscle from calsequestrin-1 knockout mice

Figure 7

Increased oxidative stress and temperature-dependent mSOF production in muscle from CASQ1-null mice. (A) Compared to WT mice, muscle samples from CASQ1-null mice exhibit reduced GSH/GSSG ratio (A) (n = 6 per group). (B, C) Mean (±SEM n = 16 to 54 fibers) mSOF frequency (B) and amplitude (C) at 20°C and 37°C for FDB fibers. (D) Representative mt-cpYFP confocal image of an FDB fiber at 37°C from a WT mouse; boxed region indicates an area containing a mSOF. (E) Top: series of pseudocolor time-lapse images from the boxed region in (D); bottom, time course of mt-cpYFP (green) and TMRE (red) fluorescence for the event indicated in (D). (F) Output standard deviation map (100 images, 1.2 s/frame) of mSOF activity for the WT FDB fiber shown in (E). Events are circled in magenta. (G-I) Same as (D-F) except for a FDB fiber at 37°C from a CASQ1-null mouse. (J) Representative mSOF event registry over time for the WT fiber (left) and the CASQ1-null fiber (right). Flash frequency (F f) is indicated to the right of the corresponding registry trace. Data expressed as mean ± SEM. (*P < 0.05). Scale bars: (D, F, G, and I), 10 μm. CASQ1, skeletal isoform of calsequestrin; GSH/GSSG, reduced and oxidized glutathione; mSOF, mitochondrial superoxide flashes; mt-cpYFP, mitochondria-targeted circularly permuted yellow fluorescent protein; TMRE, tetramethylrhodamine ethyl ester; WT, wild type.

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