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Figure 5 | Skeletal Muscle

Figure 5

From: The effect of the muscle environment on the regenerative capacity of human skeletal muscle stem cells

Figure 5

Myogenic cells of donor origin within regenerated host muscles. (a-c) Representative images of transverse cryosections of Rag2-/γ chain-/C5- host muscles that had been cryodamaged and transplanted with human CD133+ cells, stained with antibodies to human lamin A/C ((a), red) and Pax7 ((b), green). Nuclei were counterstained with DAPI (blue). (c) Merged image of human lamin A/C (red), Pax7 (green) and DAPI (blue). Scale bar = 25 μm. (d) The number of Pax7+ cells, Pax7+/human lamin A/C+ and Pax7+/human lamin A/C- cells per 100 muscle fibres in cryodamaged muscles of Rag2-/γ chain-/C5- and mdx nude mice. (e) the number of Pax7+ cells, Pax7+/human lamin A/C+ and Pax7+/human lamin A/C- cells per 100 muscle fibres in irradiated+cryodamaged muscles of Rag2-/γ chain-/C5- and mdx nude mice. (f) Percentage of donor-derived myogenic cells in each injury model. *P < 0.05, **P < 0.01. Rag, recombinase-activating gene.

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