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Figure 2 | Skeletal Muscle

Figure 2

From: Wnt/β-catenin controls follistatin signalling to regulate satellite cell myogenic potential

Figure 2

Canonical Wnt/β-catenin signalling promotes myoblast fusion in a follistatin-dependent manner. (A) EDL single myofibers were cultured in floating conditions for 3 days, and immunostained for Pax7 and myogenin expression. Shown is a typical myogenic cell cluster. (B) Percentage of myogenin+ cells on myofibers following 24 h of recombinant Wnt3a, Dkk1, or control BSA protein treatments (left) and following 24 h recombinant follistatin protein treatment, siRNAs against follistatin transfection, or control scrambled siRNAs transfection. (C) Myoblasts were cultured in differentiation medium for 3 days in the presence of recombinant Wnt3a, Dkk1, follistatin, or control BSA proteins or transfected with siRNAs against follistatin and immunostained for Myh2 expression. Scale bar 25 μM. (D) Quantification of the fusion index in myogenic cultures. Changes are expressed as relative to the control (BSA treatment or siScr transfection). Data are presented as the mean ± SEM (n = 3, **P < 0.01). (E) qPCR analysis of follistatin expression (left) and quantification of the fusion index (right) of myoblasts cultured in differentiation media for 24 h treated with siScr and BSA, siScr and Wnt3a, or siFst and Wnt3a. Data are presented as the mean ± SEM (n = 3, *P < 0.05, **P < 0.01), normalized to GAPDH.

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