Figure 4
From: Wnt/β-catenin controls follistatin signalling to regulate satellite cell myogenic potential
Canonical Wnt signalling promotes precocious differentiation in vivo. (A) Experimental time-course for in vivo muscle injury. Mice undergo direct muscle injections of BIO 2 and 4 days post-CTX injury. (B) qPCR analysis of myogenin, follistatin, and Myh2 expression in TA muscle 7 days following CTX injury. Data are presented as the mean ± SEM (n = 3, **P < 0.01), normalized to GAPDH. (C) Representative cryosections of TA muscle 7 days following CTX injury and treatment with GSK3β inhibitor (BIO) or DMSO control. Scale bar 50 μm. (D) Quantification of muscle fiber caliber in TA muscles 7 days following CTX injury and BIO treatment. Data are presented as the mean ± SEM (n = 3, *P < 0.05).