Skip to main content
Figure 4 | Skeletal Muscle

Figure 4

From: The endogenous molecular clock orchestrates the temporal separation of substrate metabolism in skeletal muscle

Figure 4

Characterization of iMS-Bmal1 −/− mice. Recombination assay (A) of genomic DNA isolated from muscle and non-muscle tissues from tamoxifen-treated (iMS-Bmal1 −/−) and vehicle-treated (iMS-Bmal1 +/+) mice at 17 to 18 weeks of age (5 weeks postinjection). Recombination of the Bmal1 gene (exon 8) yields a 572-bp PCR product. The non-recombined allele is detected at 431 bp. Western blot (B) analysis of BMAL1 expression in iMS-Bmal1 −/− and iMS-Bmal1 +/+ liver and gastrocnemius samples. Note that the original blot containing both muscle and liver samples was cut, and brightness/contrast was altered to enhance the visibility of Bmal1 in the muscle samples. (C) Real-time PCR results of time-course expression values for Bmal1, Rev-erbα, and Dbp in the iMS-Bmal1 +/+ (black) and iMS-Bmal1 −/− (red). Representative wheel running rhythms (D) of iMS-Bmal1 −/− and iMS-Bmal1 +/+ mice. White and black bars (top) indicate light and dark phases. 12 L/12D represents the 12-h light/12-h dark cycle. 12D/12D represents constant darkness conditions. Tick marks indicate wheel running activity. Representative chi-squared periodograms (E) of iMS-Bmal1 −/− and iMS-Bmal1 +/+ mice indicating approximate 24-h period lengths in both mice.

Back to article page