Figure 2
From: In vitro stability of therapeutically relevant, internally truncated dystrophins
Gel analysis of purified recombinant proteins. (A) Representative Coomassie-stained gels with 5 μg of exon-skipped dystrophins, gene therapy dystrophins, and utrophins loaded for comparison. (B) Western blot of purification fractions from tandem purification of dual-tagged full-length human dystrophin with N-terminal (NT) FLAG-tag (green channel) and C-terminal (CT) Strep-tag (red channel); fractions from Strep affinity purification and FLAG affinity purifications: load (L), void (V), wash (W), and elute (E). hDys - human dystrophin, hUtr - human utrophin.