Table 1 Biophysical properties of human dystrophin and utrophin constructs
From: In vitro stability of therapeutically relevant, internally truncated dystrophins
Construct | Molecular weight (kDa) | CD Tm1 (°C) | CD Tm2 (°C) | DSF Tm (°C) | % Aggregation |
|---|---|---|---|---|---|
hDys | 427 | 48.1 ± 1.17 | - | 45.7 ± 2.62 | 17.6 ± 7.11 |
Δex43-44 | 414 | 46.7 ± 0.70 | - | 44.3 ± 0.35 | 3.4 ± 0.73* |
Δex44-45 | 413 | 51.7 ± 1.51 | - | 48.1 ± 1.54 | 15.7 ± 2.83 |
Δex45-46 | 414 | 48.4 ± 2.38 | - | 46.0 ± 2.60 | 27.9 ± 5.28 |
Δex45-51 | 385 | 47.9 ± 4.07 | - | 44.6 ± 1.63 | 10.9 ± 7.00 |
Δex51-52 | 414 | 47.2 ± 0.87 | - | 43.8 ± 0.31 | 12.9 ± 6.70 |
Δex51-63 | 351 | 48.6 ± 3.08 | - | 43.7 ± 1.15 | 10.4 ± 7.11 |
μH2 | 139 | 47.3 ± 1.25 | 85.1 ± 4.14 | 46.0 ± 1.28 | 44.4 ± 10.10** |
μH3 | 137 | 49.5 ± 1.78 | 79.0 ± 2.82 | 45.8 ± 0.86 | 31.7 ± 6.72** |
Δ3990 | 154 | 56.1 ± 1.97** | - | 50.4 ± 1.91* | 11.7 ± 3.97 |
ΔH2-R15ΔCT | 242 | 53.7 ± 1.51 | 72.9 ± 4.11 | 49.8 ± 0.54* | 36.2 ± 1.95** |
ΔR3-15,18-23 ΔCT | 146 | 45.8 ± 2.96 | - | 47.3 ± 0.07 | 36.7 ± 3.96** |
hUtr | 399 | 46.2 ± 1.42 | - | 43.4 ± 0.36 | 12.5 ± 1.40 |
μH2 hUtr | 138 | 47.0 ± 1.16 | - | 42.9 ± 0.25 | 25.6 ± 3.58 |