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Fig. 1 | Skeletal Muscle

Fig. 1

From: Akt-mediated phosphorylation controls the activity of the Y-box protein MSY3 in skeletal muscle

Fig. 1

MSY3 is progressively dephosphorylated and accumulates in the nuclei in skeletal muscle after birth. a Left: Western blot with anti-MSY3 (ZONAB) protein extracts of limb and TA muscle at different postnatal days (pn). α-tubulin was used as normalizer. Right: densitometry calculations for MSY3 faster (dephosph) and slower (phosph) migration bands of the Western blot on the left, normalized by α-tubulin. Figure displays results representative from three independent experiments. *P <0.01 by Student’s t test. b Western blot with extracts from 15 dpc embryo limbs treated with Antarctic Phosphatase (AP), 1 unit and 5 units, and probed with anti-MSY3 Ab (ZONAB). c Western blots of nuclear and cytosolic fractions of protein extracts of limb muscle at fetal (15 dpc), postnatal (pn), and mature, 1 month (1 M), stages. Histone H3 was used as normalizer of the nucleic fraction and GAPDH as normalizer of the cytosolic fraction. d Left: expression of MSY3 evaluated by IF with anti-MSY3 Ab, ZONAB, in cross sections of limbs of 2-day-aged pups (2 pn) and TA of 1-month-aged mice (1 M). The images merge MSY3 expression (red), caveolin 3 expression (green), and Hoechst nuclei staining (blue). MSY3 localization in nuclei is labeled in purple. Scale bars = 100 μm. On the right, high magnification images (corresponding to dotted white squares) show staining of few muscle fibers. Scale bar = 40 μm. White arrows indicate sporadic MSY3 nuclear accumulation in 2 pn limb cross sections. Right Graph quantifies MSY3 positive nuclei over the total nuclei in cross sections of limbs of 2-day-aged pups (2 pn) and TA of 1-month-aged mice (1 M)

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