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Fig. 5 | Skeletal Muscle

Fig. 5

From: Akt-mediated phosphorylation controls the activity of the Y-box protein MSY3 in skeletal muscle

Fig. 5

Akt phosphorylates MSY3 in C2C12 cells, decreasing its binding activity and increasing myogenin expression. a Chromatin immunoprecipitation (ChIP) analysis for control IgG and MSY3 antibodies used individually to enrich fixed chromatin from undifferentiated (GM) or differentiated (DM) C2C12 cells, treated (+) or not (−) with LY2940002 (LY). qRT-PCR is used to quantify sequences from the myogenin promoter. b Measurements of endogenous myogenin expression by qRT-PCR in mock transfected C2C12 (−MSY3, −Akt) and MSY3 over-expressing C2C12 multiclones transfected (+MSY3, +Akt) or not (+MSY3, −Akt) with myristoylated Akt in proliferation medium (GM) or upon different times cultured in differentiation medium (24–72–96 h DM). GAPDH was used to normalize expression levels

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