Fig. 1
From: A differential response to newt regeneration extract by C2C12 and primary mammalian muscle cells
Inhibition of muscle differentiation in C2C12 cells treated with newt extract. a C2C12 myoblasts treated with 0.3 mg/ml of newt extract in either differentiation medium (DM) or growth medium (GM) showed impaired differentiation and reduced numbers of MHC-positive cells. b The percentage of MHC-positive cells was calculated by adding all MHC-positive cells (myotubes and myocytes) and dividing by the total number of cells. The percentage of MHC-positive cells was reduced in extract-treated cells in both DM and GM. Scale bar = 200 μm. *p < 0.0001