Fig. 2

Inhibition of differentiation in primary myoblasts treated with newt extract. a Primary myoblasts treated with 0.3 mg/ml of newt extract for 72 h showed reduced numbers of myotubes. b The percentage of myosin heavy chain (MHC)-positive cells (see Fig. 3b for method of calculation) was reduced in extract-treated cells. c Fusion index was reduced in primary myoblasts treated with 0.1 mg/ml or 0.3 mg/ml of extract. The degree of reduction was related to the concentration of the extract. Fusion index was calculated as the number of nuclei within multinucleated myosin-positive cells divided by the total number of nuclei × 100. Scale bar = 200 μm. *p < 0.05