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Table 1 Peptides, transitions, and instrument parameters for myostatin, propeptide, GASP-1, and FLRG

From: Myostatin as a mediator of sarcopenia versus homeostatic regulator of muscle mass: insights using a new mass spectrometry-based assay

  Parent mass Product mass Collision energy S-lens
Myostatin
IPAMVVDR 450.68 619.33 19 123
  450.68 690.40 17 123
  450.68 787.52 17 123
 IIYGKIPAMV*VDRCGCS 453.65 619.44 19 123
  453.65 690.50 18 123
  453.65 763.65 16 123
 EQIIYGK 425.66 480.31 13 86
  425.66 593.47 14 86
 EQ*IIYGK 429.14 480.35 13 86
  429.14 600.43 14 86
Propeptide
TVLQNWLK 501.18 560.32 18 131
  501.18 688.60 18 131
  501.18 801.78 18 131
 SIDVKTVLQNW*LKQPESN 504.75 567.25 19 131
  504.75 695.42 17 131
  504.75 808.65 15 131
 ELIDQYDVQR 639.70 680.42 21 132
  639.70 808.50 20 132
  639.70 923.77 20 132
 ELIDQYD*VQR 642.92 686.48 20 132
  642.92 814.36 22 132
  642.92 929.43 21 132
GASP-1
ADFPLSVVR 502.28 670.53 18 131
 AGVLRADFPLSV*VRGHQAA 505.23 579.46 19 131
  505.23 676.64 16 131
 VSELTEEPDSGR 659.81 789.36 20 168
  659.81 890.51 21 168
 VSELTEE*PDSGR 662.73 795.31 21 168
  662.73 896.60 21 168
FLRG
LQVCGSDGATYR 663.78 826.30 23 168
  663.78 986.34 21 168
  663.78 1085.46 21 168
 GLPARLQVCGSD*G*ATYRDECEL 666.78 832.56 24 168
  666.78 992.69 23 168
  666.78 1092.15 22 168
  1. Target peptides for each protein, parent and product ion masses as well as MS parameters and transitions used quantitation of each protein are indicated in bold and/or italics. Labeled amino acids (13C5 15N-V, 13C6 15N-L, 13C5 15N-P, 13C2 15N-G, and 13C3-A) are indicated by the asterisk