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Fig. 2 | Skeletal Muscle

Fig. 2

From: Upregulated IL-1β in dysferlin-deficient muscle attenuates regeneration by blunting the response to pro-inflammatory macrophages

Fig. 2

Ongoing satellite cell proliferation and macrophage infiltrate in uninjured Bla/J muscle. a Immunofluorescence staining of uninjured 3-month-old WT and Bla/J skeletal muscle. Upper panels mice were treated with BrdU in the drinking water for 7 days. Frozen muscle sections were immunostained with anti-laminin (blue) and anti-BrdU (green) antibodies. Nuclei were detected by propidium iodide (PI, red). Bottom panels muscle sections were immunostained with an anti-F4/80 (green) antibody and nuclei were detected by DAPI staining (blue). b Proliferating cells quantitated in WT and Bla/J muscle are shown as percent of myonuclei that are BrdU-positive (proliferating). n = 3 non-overlapping fields from three mice. c Quantitation of the number of F4/80-positive macrophages in muscle per mm2. n = 3 non-overlapping fields each from three mice. d Expression of M1-specific markers using qRT-PCR in WT (black) and Bla/J (white) uninjured muscle. e Expression of M2-specific markers in WT (black) and Bla/J (white) muscle using qRT-PCR. n = 3 mice per data point. Data are shown as means ± SEM. *P < 0.05; **P < 0.01. Scale, 50 μm

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