Fig. 3
Response to injury is attenuated in Bla/J muscle compared with WT. a H&E staining of WT (left) and Bla/J (right) muscle on day 7 after injury. b Fiber cross-sectional areas (CSA) from WT (black) and Bla/J (white) on day 7 after injury. Fiber diameter distribution of Bla/J muscle is shifted to the left. Residual tattoo ink (blue staining) can still be seen in the injured Bla/J muscle. c Immunofluorescence staining for BrdU (green) and laminin (blue) on indicated days following notexin injury in WT or Bla/J TA muscle. Nuclei were detected with propidium iodide (PI, red). d Quantitation of BrdU-positive nuclei on day 11 after injury shown as percent of BrdU-positive cells over total myonuclei. e Immunofluorescence staining for F4/80 (green) on indicated days following notexin injury in WT or Bla/J TA muscle. Nuclei were detected with DAPI (blue). f Number of F4/80-positive macrophages per mm2 on indicated days after injury. n = 3. Expression of M1-specific markers IFNγ (g) and TNFα (h) and M2-specific markers Arg-1 (i) and YM1 (j) using qRT-PCR on indicated days following injury. Data are shown as means ± SEM. *P < 0.05. Scale bar, 50 μm