Fig. 7

NFκB and IL-1β in myoblast-macrophage co-cultures. a Representative Western blot showing phosphorylation of the NFκB p65 subunit in WT or A/J myoblasts either cultured alone (-), or co-cultured for 3 days with Mϕ, or M1 macrophages. b Quantitation of phospho-p65 pixel intensity in a, normalized to vinculin pixel intensity. n = 3 independent experiments. c IL-1β ELISA analysis of whole cell lysates prepared from WT (black) or A/J myotubes (white) co-cultured for 24 h with M1 or M2a macrophages. d MSD IL-1β assay performed on supernatants from WT (black) or A/J myotubes (white) co-cultured for 24 h alone, with M1 or M2a macrophages. Two-way ANOVA with Tukey post hoc test were used to calculate P values. n = 3 cultures per data point. Data are shown as means ± SEM. *P < 0.05; ***P < 0.001