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Fig. 8 | Skeletal Muscle

Fig. 8

From: Upregulated IL-1β in dysferlin-deficient muscle attenuates regeneration by blunting the response to pro-inflammatory macrophages

Fig. 8

IL-4 potentiates muscle differentiation in WT and A/J myoblasts. a Immunofluorescence staining of differentiated WT and A/J cultures treated for 72 h with 4 or 20 ng/ml of IL-4. Control cultures were treated with equal volumes of PBS (NT). Following incubation, cultures were immunostained with anti-MyoD (green) and anti-MyHC (red). Scale bar, 50 μm. b Myofusion index (expressed as % fusion). ce Gene expression of MyoD (b), Myf5 (c), and myogenin (d) determined by qRT-PCR in WT and A/J cultures treated with indicated concentrations of IL-4. N = 4. Data are shown as means ± SEM. ANOVA with Tukey post hoc analysis, *P < 0.05; **P < 0.01; ***P < 0.005

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