Fig. 5
From: Regions of ryanodine receptors that influence activation by the dihydropyridine receptor β1a subunit
ASI residues enhance the effect of β1a on recombinant RyR1 channel activity in lipid bilayers. a, b Three second (3 s) traces of ASI(+)RyR1 (a) or ASI(−)RyR (b) activity at +40 mV, opening upwards from the closed (c) to maximum open (o) level, before (top panel; control, cis 10 μM [Ca2+], no ATP) and after addition of 10 nM β1a subunit (middle panel) or 50 nM β1a subunit (bottom panel) to the cis chamber. c Average relative P o (log10 rel P o ) were calculated in the same ways as described for averaged relative P o in Fig. 2a, left. d Average P o . c and d Single channel parameters were calculated from ~180 s of channel activity (at +40 and −40 mV). Data in d is shown for 0 nM β1a (black bar), 10 nM β1a subunit (dark grey bar), and 50 nM β1a subunit (light grey bar). Error bars indicate + SEM, n = 9–12 experiments/bar. *p < 0.05 vs control or 0 nM β1a subunit determined using paired (c) or un-paired (d) Student’s t-test, # p < 0.05 vs 10 nM β1a subunit on RyR1 ASI(+) determined by ANOVA