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Fig. 4 | Skeletal Muscle

Fig. 4

From: Intracellular Ca2+-handling differs markedly between intact human muscle fibers and myotubes

Fig. 4

The localization of Ca2+-handling proteins differs markedly between human muscle fibers and myotubes. a Representative immunofluorescent staining for RyR and DHPR in human muscle fibers (top row) and myotubes (middle row). b Magnification of the area indicated in a. c, d Fluorescent intensity (a.u.) for RyR (black) and DHPR (red) in a human muscle fiber (left) and myotube (right). e Representative immunofluorescent staining for SERCA1 and SERCA2 in human muscle fibers (top row) and myotubes (middle row). f Magnification of the area indicated in e. Scale bars indicate 10 μm. Primary antibody was omitted for the respective negative controls (bottom row in a, b and e, f)

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