Fig. 4

Effects of adiponectin on markers of the myogenic program in mdx mice. The expression of MyoD (a) and Myf5 (b), two myogenic regulatory factors, was analyzed by Western blotting in tibialis anterior muscles from the three groups of mice. Levels of each marker were normalized to actin levels. mRNA levels of Mrf4 (c) and myogenin (d), two markers of muscle differentiation. e mRNA levels of Myh3 (eMyHC), a marker of skeletal muscle regeneration. mRNA levels were normalized to cyclophilin and the subsequent ratios were presented as relative expression compared with WT values. f Tibialis anterior sections stained with hematoxylin-erythrosin-safran. Scale bar = 100 μm. g The percentage of muscle fibers with central nuclei was counted. Results are means ± SD; n = 6 mice per group. **p < 0.01; ***p < 0.001 vs. WT; ##p < 0.01; ###p < 0.001 vs. mdx mice