Involvement of adiponectin in the pathogenesis of dystrophinopathy

Skeletal Muscle

Table 1 Characteristics of mdx mice overexpressing adiponectin

	WT	mdx	mdx-ApN
Body weight (g)	22.2 ± 0.6	23.1 ± 0.7	22.2 ± 0.9
Tibialis anterior muscle pair weight (mg)	72 ± 2	92 ± 6**	76 ± 5***
Plasma ApN (μg/ml)	4.2 ± 0.2	2.6 ± 0.2**	3.6 ± 0.1^/**
Adipose tissue ApN (mRNA levels)	1.0 ± 0.2	0.5 ± 0.1**	1.4 ± 0.2^/**

Three groups of mice were compared at the age of 8 wks: the mdx-ApN mice (mdx mice overexpressing ApN), their mdx littermates (true controls), and wild-type (WT) mice. Blood, tibialis anterior muscle and inguinal adipose tissue were sampled between 09.00 and 11.00 h. ApN was measured in plasma by ELISA, and in adipose tissue by RT-qPCR. In this case, ApN gene expression was normalized to the levels of cyclophilin and presented as relative expression compared to WT values. Data are means ± SD for six mice per group
*p < 0.01; **p < 0.001 compared to WT; ***p < 0.001 compared to mdx

ISSN: 2044-5040