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Fig. 2 | Skeletal Muscle

Fig. 2

From: GRAF1 deficiency blunts sarcolemmal injury repair and exacerbates cardiac and skeletal muscle pathology in dystrophin-deficient mice

Fig. 2

GRAF1 regulates skeletal and cardiac muscle membrane repair. a Graf1Wt/Wt and Graf1Gt/Gt primary myotubes were treated with FM 1-43 membrane dye (red) for 5 min and imaged prior to confocal-directed laser injury (1 μM × 0.5 μM within red square; top panels) and for 3 min post-injury. Note the pronounced FM 1-43 accumulation in Graf1Gt/Gt cells. b Quantification of dynamic FM 1-43 dye accumulation following injury (n = 23–28 cells per genotype; N = 5). Data are represented as mean ± sem c Quantification of FM 1-43 dye-positive cardiomyocytes treated with or without saponin following transfection with control (GFP) or Graf1-specific siRNAs. Note the significant increase in dye accumulation in GRAF1-depleted cells. Western blot inset demonstrates efficient Graf1 knockdown. γ-tubulin is shown as the loading control

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