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Fig. 3 | Skeletal Muscle

Fig. 3

From: GRAF1 deficiency blunts sarcolemmal injury repair and exacerbates cardiac and skeletal muscle pathology in dystrophin-deficient mice

Fig. 3

GRAF1 is required for optimal cardiomyocyte membrane repair in vivo. a Representative images of Evans blue dye (EBD; red) uptake in the hearts from Graf1Wt/Wt and Graf1Gt/Gt adult mice prior to (top panels) and 24 h after subjection to intra-cardiac injection of cardiotoxin (CTX) (middle panels) or i.p. injection of isoproterenol (ISO) (bottom panels). Cardiomyocytes and nuclei are counterstained with troponin T antibody (green) and DAPI (blue), respectively. b Graphical representation of the area of EBD uptake in the left ventricle (LV) cross-section of injured hearts. c Quantification of serum troponin levels in injured mice. Note the significant increases in markers of cardiomyocyte damage in GRAFgt/gt mice. (N = 6–7 mice per genotype). Data are represented as mean ± sem. Scale bars = 20 μm

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