Fig. 7
Histopathological analysis of diaphragm muscles in GRAF1/dystrophin-deficient mice. a Diaphragm cross-sections from 6-month-old male mice with indicated genotypes stained with hematoxylin and eosin (H&E) (top panels) or Sirius red and imaged under polarized light to visualize fibrosis (bottom panels). b Quantification of average diaphragm thickness from 6-month-old male mice. c Graphical representations of collagen composition of diaphragms from 6-month-old male mice (refer to methods for quantification details; *p < 5 × 10−4; **p < 0.01; # p < 0.05; N = 5 mice per genotype). Data are represented as mean ± sem. Black scale bars = 25 μm; white scale bars = 100 μm