Fig. 1

Detection of the locus targeted for deletion in homozygous Dysf ^tm1Kcam mice. a Schematic drawing of the wild-type locus and the expected deletion. The arrows depict the two homologous arms used for targeted deletion. b Results of a genotyping PCR using DNA from toe biopsies of wild type, heterozygous mutant (Dysf ^tm1Kcam/+), and homozygous mutant (Dysf ^{tm1Kcam/tm1Kcam} ) mice, as well as a no template control (NTC). As expected, PCR to detect the targeting vector was negative for wild type mice, and positive for heterozygous and homozygous Dysf ^tm1Kcam mice. However, PCR to amplify the sequence encompassing exons 51–54, which had been targeted for deletion in generating the Dysf ^tm1Kcam line, was positive in all mice, suggesting that homologous recombination had failed to delete the targeted region