Fig. 2
From: Skeletal muscle interleukin 15 promotes CD8+ T-cell function and autoimmune myositis
IL-15 is presented on the surface of skeletal muscle cells. a Expression of IL-15 and IL-15Rα on C2C12 myoblasts. Cells were treated with TNF-α and IFN-γ (TNF + IFN) or without cytokine (Control) for 24 h and then stained with anti-IL-15 and IL-15Rα antibodies for flow cytometry analysis. “Unstained” indicates cells without antibody staining. Data are representative of three independent experiments with similar results. b Quantification of IL-15 bound by cell surface IL-15Rα on C2C12 myotubes. C2C12 myotubes were first treated with TNF-α and IFN-γ for 24 h, incubated with acid glycine buffer to dissociate IL-15 from cell surface IL-15Rα, and then re-treated with exogenous IL-15. Cell lysates from each step were collected and the amount of IL-15/ IL-15Rα complex was measured by ELISA. Data are mean ± SEM of triplicates and representative of two independent experiments with similar results. ***p < 0.001