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Fig. 5 | Skeletal Muscle

Fig. 5

From: Skeletal muscle interleukin 15 promotes CD8+ T-cell function and autoimmune myositis

Fig. 5

Skeletal muscle cells presented antigen and provided IL-15 to promote the effector function of CD8+ T cells under TNF-α and IFN-γ treatment. a Expression of IL-15 and H-2Kb on parental C2C12 and C2C12-Kb/OVA cells. Cells were treated with TNF-α and IFN-γ (TNF + IFN) or without (Control) for 24 h, stained with anti-IL-15 or anti-H-2Kb antibody, then analyzed by flow cytometry. “Unstained” indicates C2C12 or C2C12-Kb/OVA myoblast without antibody staining. b Expression of grB and IFN-γ by CD8+ T cells co-cultured with cytokine-treated C2C12-Kb or C2C12-Kb/OVA myoblasts for 8 h. After co-culture, CD8+ T cells were stained with anti-grB and IFN-γ antibody by intracellular staining then analyzed by flow cytometry. Data are representative of three independent experiments with similar results. c Effect of anti-IL-2 (αIL-2, S4B6) or anti-IL-2/15Rβ (αRβ, TM-β1) antibody on grB and IFN-γ production by CD8+ T cells co-cultured with TNF + IFN-pretreated C2C12-Kb/OVA myoblasts. The percentages of grB+ and IFN-γ+ cells in CD8+ T cells are indicated in the dot plots. Data from independent experiments were compiled as % normalized activation using the percentage of grB+ or IFN-γ+ cells in CD8+ T cells of the TNF + IFN-pretreated C2C12-Kb/OVA cell co-culture group of each experiment as 100 % (bottom panels). Isotype controls, Rat IgG2a (Iso) and Rat IgG2b (Iso). Each symbol is representative of one independent experiment. **p < 0.01, ***p < 0.001

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