Skip to main content
Fig. 3 | Skeletal Muscle

Fig. 3

From: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells

Fig. 3

BMP4-treated hiPSCs/hESCs express dystrophin protein. a Western blot in three pluripotent stem cell lines (hiPSCs 2, DMD hiPSCs 2, and hESCs 1) at day 4 either without or after BMP4 treatment. b Western blot in hiPSCs 1 and hESCs 1 from days 3 through 7 following BMP4 treatment. c Western blot analysis on protein extracts from hiPSCs 1 4 days after BMP4 treatment, from a BMD patient muscle biopsy with an exon 2 frameshift mutation (c.40_41del (p.Glu14ArgfsX17)) and from normal skeletal muscle. d Immunolabeling of hiPSCs 1 and DMD hiPSCs 2, 4 days after BMP4 treatment. Scale bar = 20 μm. (Dystrophin antibody: DYS1 (RRID: AB_442080) for Western blots and immunolabeling; muscle biopsy protein extract from a healthy individual serves as a control; α-tubulin was used as loading control)

Back to article page