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Fig. 5 | Skeletal Muscle

Fig. 5

From: Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells

Fig. 5

Dp412e is expressed in embryoid bodies (EBs). a Quantitative RT-PCR of Dp412e transcripts in hiPSCs 1 and hESCs 1 EBs from day 0 to day 10 of differentiation with or without BMP4 treatment. Gene expression was normalized to PPIA and plotted (log10 scale) relative to each cell line control at D0. b Western blot analysis on hESCs 1, hiPSCs 1, and DMD hiPSCs 2 EBs from day 3 to day 14 without BMP4 treatment. c Western blot analysis on hiPSCs 1 and DMD hiPSCs 2 EBs from day 0 to day 14 with BMP4 treatment. (Dystrophin antibody: DYS1; BMP4-treated hiPSCs 1 in 2 dimensions (2D) culture (4 days after treatment) was used as control; α-tubulin was used as loading control)

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