Fig. 1From: Investigating mechanisms underpinning the detrimental impact of a high-fat diet in the developing and adult hypermuscular myostatin null mouseEffect of high fat on embryonic muscle development. EDL muscle morphology at developmental stage E18.5 from wild type (WT) and myostatin null (Mstn−/−) embryos from mice subjected to maternal normal (ND) or a high-fat (HF) diet. a Representative immunofluorescence images depicting primary (slow; red) and secondary (fast; green outline) myotubes from EDL. Scale 100 μm. b Total myotube number in EDL muscle at E18.5. c Myotube cross-sectional area and d myotubes with central nuclei per unit area. e, f Total myotube number of the TA and soleus muscles at E18.5 from WT and Mstn−/− embryos from mothers under a ND or HF diet. ANOVA; (*) P < 0.05 vs. WT ND; (#) P < 0.05 vs. Mstn−/− ND. Data are from N = 6–8 embryos per groupBack to article page