Fig. 2
From: Differential myofiber-type transduction preference of adeno-associated virus serotypes 6 and 9
Analysis of GFP and myofiber-type correlation in AAV6- or AAV9-transduced muscles. a Images of representative sections after immunohistochemistry with four antibodies to MyHC isotypes (staining of each MyHC isotype separately is shown in Additional file 1: Figure S5) and GFP fluorescence in a consecutive section. Examples of matching myofibers are marked with arrowheads. Scale bar in 50 μm. b Plots show mean fluorescent intensity (MFI) distribution of MyHC isotypes and GFP within a myofiber (N AAV6 = 188 fibers; N AAV9 = 202 fibers); GFP in light green, MyHC-2b in dark green, MyHC-2a in red, and MyHC-1 in blue. Myofiber types that predominantly express MyHC-2b, MyHC-2a or MyHC-2a/1, and negatively stained myofibers are schematically indicated. c Scatter plots show the distribution of observed GFP MFI versus predicted GFP in AAV6 (in green) or AAV9 (in blue) conditions. Linear regression and fitness (R 2) are depicted in green or blue to AAV6 or AAV9, respectively. Predicted GFP is calculated using MyHC-2b, MyHC-2a, and MyHC-1 isotypes are variable (left) or MyHC-2b, MyHC-2a, and MyHC-1 isotypes and CSA (right)