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Fig. 1 | Skeletal Muscle

Fig. 1

From: Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice

Fig. 1

No myonuclear accretion with wheel-running in adult mouse plantaris muscles irrespective of satellite cell depletion. a–b Pax7 immunohistochemistry was performed on plantaris muscle cross sections (7 μm) from vehicle (a)- and tamoxifen (b)-treated animals to detect the presence of satellite cells (green). Sections were counterstained with DAPI (blue) and Pax7+/DAPI+ nuclei were quantified (white arrowheads). Scale bar = 100 μm. c Quantification of Pax7+/DAPI+ nuclei. Data are represented as Pax7+ nuclei per fiber. (d) Myonuclear number was assessed in plantaris muscle cross sections that were immunohistochemically analyzed for dystrophin (red) and co-stained with DAPI (blue). DAPI+ nuclei residing within the dystrophin boundary are classified as myonuclei (white arrows). Interstitial nuclei are denoted with green arrows. A representative image from an ambulatory vehicle muscle is shown. Scale bar = 50 μm. e Quantification of myonuclei represented as myonuclei per muscle fiber. Asterisk indicates a significant effect of tamoxifen between condition-matched groups. All values are presented as mean ± SE. Significance was set at p ≤ 0.05

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Skeletal Muscle

ISSN: 2044-5040

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