Skip to main content
Fig. 6 | Skeletal Muscle

Fig. 6

From: Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice

Fig. 6

No detectable Cre recombination or depletion of pax7+ nuclei in response to tamoxifen in brain tissue. To verify that tamoxifen was not depleting Pax7+ cells in the brain, and consequently causing centrally mediated effects, Pax7-ZsGreen reporter mice and Pax7/DTA mice were treated with tamoxifen and assessed for either the presence of ZsGreen positive nuclei or a depletion of Pax7+ nuclei, respectively. Representative images of brain (a–b) and tibialis anterior muscles (c–d) from the same ZsGreen animals were immunohistochemically probed for Pax7 (red nuclei). White arrows indicate Pax7+ nuclei in tibialis anterior muscles from both vehicle-treated (c) and tamoxifen-treated animals (d). Tamoxifen-treated muscles have GFP+ labeled Pax7 + cells (red/green overlay in d) indicating tamoxifen-induced recombination which is notably absent in both the vehicle-treated ZsGreen muscle and in both the vehicle (a)- and tamoxifen (b)-treated ZsGreen brain sections. Representative images of brain from Pax7/DTA mice (e–f) immunohistochemically probed for Pax7 (red nuclei). Scale bars = 100 μm

Back to article page

Skeletal Muscle

ISSN: 2044-5040

Contact us