Pervasive satellite cell contribution to uninjured adult muscle fibers

Skeletal Muscle

Table 1 Quantification of tdTomato recombination

Percentage of tdTomato+ SCs	8 weeks	12 weeks	27 weeks	All time points
EDL	100.0	95.2	100.0	98.7
Sol	94.7	100.0	94.4	97.1
EOM	95.0	100.0	96.6	97.1
Gastroc	100.0	97.1	100.0	99.4
TA	96.5	97.4	100.0	97.6
Diaphragm	100.0	100.0	96.7	99.0

Cross-sections of indicated muscle with recombination induced at 8, 12, or 27 weeks were stained for Pax7, laminin, and counterstained with DAPI to mark nuclei. The number of tdTomato-expressing Pax7+ satellite cells was counted and divided by the total number of Pax7+ satellite cells. Between 20 and 80 satellite cells were counted for each experimental time point per muscle. A total of 652 satellite cells were counted. Three to six biological replicates were analyzed per time point

ISSN: 2044-5040