Fig. 6

Normal cardiac phenotype in 18-month-old Ano5 KO mice after I.P. injection of 100 mg/kg ISO for 14 days. a Representative serial M-mode echocardiography in WT and Ano5 KO mice measured before and 14 days after ISO treatment. b H&E-stained cross sections of WT and Ano5 KO mice hearts subjected to PBS or ISO for 2 weeks. c The ratio of heart/body weight in WT and Ano5 KO mice measured before and 14 days after ISO treatment. d Quantification of mean cross-sectional area of cardiomyocyte from WT and Ano5 KO mice. (e, f) Graph representing the mean fractional shortening (FS) and ejection fraction (EF) as measured by M-mode echocardiography in WT and Ano5 KO mice before and 14 days after ISO treatment. Scale bar = 20 μm. ∗p < 0.05 versus WT controls. ns, no statistical significance. The number of mice is 5–8 for each group