Table 1 Antibodies used in immunohistochemistry
From: Prenatal muscle development in a mouse model for the secondary dystroglycanopathies
Antibodies | Manufacturer | Dilution | Species |
|---|---|---|---|
α-dystroglycan (IIH6) | Millipore | 1:200 | Mouse (IgM) |
β-dystroglycan | Vector laboratories | 1:20 | Mouse |
Pax7 | Developmental Studies Hybridoma Bank (DSHB) | 1:50 | Mouse |
Ki67 | DAKO | 1:200 | Rat |
Type IV collagen | Invitrogen | 1:50 | Goat |
Perlecan | Invitrogen | 1:5000 | Rat |
Pan Laminin | Sigma | 1:200 | Rabbit |
Laminin α1 | Gift from Professor Madeleine Durbeej-Hjalt | 1:100 | Rat |
Laminin α2 | Enzo | 1:200 | Rat |
Laminin α4 | R&D systems | 1:200 | Goat |
Laminin α5 | Gift from Madeleine Durbeej-Hjalt | 1:200 | Rabbit |
Laminin β1 | Millipore | 1:2000 | Rat |
Laminin γ1 | Millipore | 1:2000 | Rat |
Fast myosin heavy chain | Leica | 1:20 | Mouse |
Slow MHC | Millipore | 1:200 | Mouse |
Developmental MHC | Leica | 1:20 | Mouse |
Mandys1 (dystrophin) | Gift from Professor Glenn Morris | 1:20 | Mouse |
Myogenin | Santa Cruz | 1:200 | Rabbit |
MyoD | Santa Cruz | 1:200 | Rabbit |