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Fig. 4 | Skeletal Muscle

Fig. 4

From: Chromatin-wide and transcriptome profiling integration uncovers p38α MAPK as a global regulator of skeletal muscle differentiation

Fig. 4

ChIP-on-chip analysis for p38α in myogenic cells. Chromatin immunoprecipitation (ChIP) was performed in C2C12 myoblasts (under different culture conditions) using a p38α-specific antibody. a Non-proportional 2-way Venn diagram showing the number of promoters bound by p38α in the two experimental conditions: proliferation (GM) and 24 h of differentiation (24 h DM). b Clustering analysis of genes bound by p38α in GM and 24 h DM. Genes were grouped in five clusters according to the pattern of binding along the promoter. Cluster 1 is enriched in transcription factors, developmental regulators, and muscle-related genes. Cluster 2 is enriched in genes involved in bone development, developmental regulators, and transcription factors and cluster 3 includes miscellaneous genes. Cluster 4 is enriched in sensory perception and olfactory receptors whereas cluster 5 is enriched in protein transport, phosphorylation, and cell cycle-related genes. c Functional annotation analysis of the Gene ontology (GO) of bound genes in GM and 24 h DM was performed using DAVID. The top annotation clusters are shown according to their enrichment score. Names are based on enriched GO annotations. d Table showing transcription factor binding sites of p38α-bound genes. Analyses of DNA-binding elements present in promoters were performed using GSEA. The highest P value element is shown. Unknown binding elements have been omitted

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