Fig. 1

Tamoxifen administration-induced PHD2 deletion in skeletal muscle in Phd2 ^f/f /Rosa26 ^CreERT2 mice. a . PHD1, 2, and 3 deletion efficiency in the gastrocnemius muscles of tamoxifen-treated Phd2 ^f/f /Rosa26 ^CreERT2 mice was determined using qRT-PCR. Relative gene expression was determined using gastrocnemius muscle tissue cDNA (n = 3–4 mice per group). b . Anti-PHD2 and PHD3 Western blotting of gastrocnemius and soleus muscles at 6 weeks after tamoxifen administration. c . The expression of HIF-1α in gastrocnemius and soleus at 5 weeks after tamoxifen administration. d. The level of hif-1α mRNA in gastrocnemius at 5 weeks after tamoxifen administration. *p < 0.05; **p < 0.01 compared to control. Values are means ± SEM