Fig. 4
PHD2 deficiency induces slow muscle fiber type switching in the soleus and gastrocnemius muscles. a The composition of muscle fiber type was analyzed by immunostaining. Frozen sections of the soleus and gastrocnemius muscle at 6 weeks after tamoxifen treatment were stained with antibodies for MyHC I/slow (green) and MyHC IIa/fast (green) and counterstained for laminin (red). b-c The proportion of MyHC I and MyHC IIa muscle fibers was measured using ImageJ software (n = 5 per group). d-eWestern blotting confirmed the MyHC I and MyHC IIa protein levels in the soleus and gastrocnemius muscles of Phd2f/f and Phd2 cKO mice (n = 4 per group). **p < 0.01 compared to control. Values are means ± SEM (scale bar = 100 μm)