Fig. 8

The effect of FK-506 treatment on the myosin heavy chain (MyHC) composition in soleus muscle of Phd2-deficient mice. a The composition of muscle fiber type was analyzed by immunostaining after the treatment of FK-506. Frozen sections of the soleus muscle taken at 6 weeks after tamoxifen treatment were stained with antibodies for myosin heavy chain (MyHC) I/slow (green) and counterstained for laminin (red) (scale bar = 100 μm). b-c The treatment of FK-506 for 7 days at 4 week after tamoxifen administration suppressed the increase in the proportion of slow muscle fiber type in both soleus and gastrocnemius muscles (n = 4 per group). d-e The capillary density in soleus and gastrocnemius muscles was determined by detecting CD31-positive cells after FK-506 treatment using immunostaining (n = 5 per group). *p < 0.05; **p < 0.01 compared to control. Values are means ± SEM