Fig. 4

Evaluating reporters with multiple DUX4 binding sequences. a Dose-dependent activity of reporters with 1–24 copies of the CT motif at 100 ng of DNA/well. Note the logarithmic scale. b Dose-dependent activity of the same reporters at 4 ng/well. Synergy is demonstrated by the presence of activation with the 2× reporter but its absence with the 1× reporter at this concentration, as well as the continued increase in activity as copy number increases to 24×. c Transcriptional activity of reporters with two CT motifs spaced at different distances (indicated), compared to a reporter with a single CT motif