Fig. 2

TSCmKO and RAmKO mice are both resistant to a high-fat diet. a TSCmKO (n = 7) and RAmKO (n = 5) mice do not gain significant weight on a HFD when compared to control (Ctrl) mice (n = 12). b TSCmKO and RAmKO mice are resistant to HFD-induced hepatic steatosis, shown by decreased lipid accumulation in Oil Red O-stained liver (n = 3). Arrows indicate Oil Red O-stained lipids. Scale bar: 100 μm. c Twenty-week-old RAmKO mice (n = 5) show an increased glucose tolerance on a GTT after a HFD when compared to TSCmKO (n = 6) and control (Ctrl) mice (n = 12). d Twenty-week-old TSCmKO mice (n = 6) show increased insulin sensitivity on an ITT after a HFD when compared to RAmKO (n = 5) and control (Ctrl) mice (n = 12). Data presented are all from male mice of the indicated genotypes. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001