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Fig. 2 | Skeletal Muscle

Fig. 2

From: Characterization of a multiprotein complex involved in excitation-transcription coupling of skeletal muscle

Fig. 2

Isolation of protein complexes containing DHPR, P2Y2R, and Panx1 from skeletal triads using 2D blue native SDS-PAGE. a Silver staining of a mouse triad sample resolved by BN/SDS-PAGE. Several multiprotein complexes are observed as dots in a same vertical lane. bd Immunoblot for complex putative interactors in triad-enriched samples derived from rat (b), BalbC mouse (c), or C57 mouse (d) previously resolved by BN/SDS-PAGE. Co-localization in the same vertical lane (as marked with arrowheads) suggests a multiprotein complex containing DHPR, Panx1, P2Y2R, and Cav3

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