Fig. 3
Subcellular distribution of DHPR, P2Y2R, Panx1, and Cav3 in isolated skeletal fibers. a Representative confocal immunofluorescence images for putative complex constituents in mouse FDB-isolated fibers. b–e Double-immunostaining and co-localization assays for different protein complex constituents. Protein distribution at the fiber z-axis was performed by line scan and z-projection reconstruction. Quantitation of fluorescence intensity in the area marked with a dashed line is shown under each image. Scale bars = 10 μm