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Fig. 4 | Skeletal Muscle

Fig. 4

From: Characterization of a multiprotein complex involved in excitation-transcription coupling of skeletal muscle

Fig. 4

Association between DHPR, P2Y2R, Panx1, and Cav3 demonstrated by proximity ligation assay technology. a Protein-protein proximity was demonstrated using the PLA probe technology in a whole fiber, as indicated (DHPR with Panx1, DHPR with P2Y2R, DHPR with Cav3, Panx1 with Cav3, and P2Y2R with Cav3). Z-stacks were collected by confocal microscopy (35–40 slices, 1 μm each), and then pixels in all the stacks were collapsed into one single illuminated image. b Positive PLA dot distribution in the median plane of the fibers for the protein-protein proximity assessed (DHPR with Panx1, DHPR with P2Y2R, DHPR with Cav3, Panx1 with Cav3, and P2Y2R with Cav3). Images correspond to the six medial slices collapsed in a single image. Scale bar = 10 um

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