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Fig. 5 | Skeletal Muscle

Fig. 5

From: Characterization of a multiprotein complex involved in excitation-transcription coupling of skeletal muscle

Fig. 5

Characterization of an L6 skeletal cell line overexpressing Panx1-Strep-tag. a Representative immunofluorescence images of recombinant Panx1 overexpression, either in myoblast or myotube differentiation stages, using a Strep-tag antibody. Overexpression was induced with 5 mM sodium butyrate ON. b Panx1-Strep-tag mRNA levels were analyzed by qPCR using primers against the Strep epitope (n = 3). c Panx1 protein expression was assessed by immunoblot using Strep-tag antibodies. d The mRNA level of whole Panx1 (both endogenous and Panx1-strep) was analyzed by qPCR using primers against Panx1 (n = 3). e L6 cells overexpressing Panx1-Strep-tag properly differentiate to myotubes. Representative images of light microscopy are shown. In b and d, values are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001

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Skeletal Muscle

ISSN: 2044-5040

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