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Fig. 7 | Skeletal Muscle

Fig. 7

From: Characterization of a multiprotein complex involved in excitation-transcription coupling of skeletal muscle

Fig. 7

Nearness between DHPR, Panx1, P2Y2R, and Cav3 reinforced by proximity ligation assay technology. In situ proximity ligation assay (PLA) probes shown in red a well-defined closeness between DHPR and Panx1 (a), DHPR and Cav3 (b), DHPR and P2Y2R (c), Panx1 and Cav3 (d), and P2Y2R and Cav3 (e) in L6-Panx1 myotubes. No interaction was observed between DHPR and the nuclear envelope protein Lap2, as expected (f). To visualize cell limits and morphology, exogenous Panx1-Strep was stained in the same preparations using an anti-strep antibody (green). Scale bars = 10 μm

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Skeletal Muscle

ISSN: 2044-5040

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