Fig. 2

Akt/mTOR signaling is unchanged following loss of αDG glycosylation. a Representative images of littermate (Tam LC) or inducible knockout (Tam iKO) iliopsoas. H&E and αDG glyco images are shown. Scale bar = 100 μm. b Western blot analysis of solubilized skeletal muscle from hind limbs of Tam LC or Tam iKO mice. c Quantification of Akt phosphorylation at T308 and S473, mTOR phosphorylation at S2448, and S6 phosphorylation at S235/236 relative to total Akt, mTOR, and S6 protein, respectively, as a measure of protein activation. Data are presented as mean ± SEM. n = 9 per group