Fig. 6

Fibrosis is reduced in iliopsoas and diaphragm of RAPA-treated Myf5/Fktn KO mice. a Representative images from iliopsoas (top) or diaphragm (bottom) of daily study mice stained with dystrophin/ColVI; nuclear DAPI counterstain shown in blue. Scale bar = 100 μm b Quantification of muscle fibrosis, ColVI-positive area, in iliopsoas (left) or diaphragm (right) muscles, detected by immunofluorescence and counted as positive (green) pixels per tissue/total pixels per tissue. Two-way ANOVA; *p < 0.05; ***p < 0.001. Ilio: n = 9 VEH LC, n = 8 RAPA LC, n = 12 VEH KO, n = 12 RAPA KO. Dia: n = 5 VEH LC, n = 6 RAPA LC, n = 7 VEH KO, n = 7 RAPA KO